Biology, 12.09.2019 23:30 Geo777

Suppose you wanted to study genes controlling the structure of bacterial cell surfaces. you decide to start by isolating bacterial mutants that are resistant to infection by a bacteriophage that binds to the cell surface. the selection procedure is simple: spread cells from a culture of sensitive bacteria on a petri plate, expose them to a high concentration of phages, and pick the bacterial colonies that grow. to set up the selection you could (1) spread cells from a single liquid culture of sensitive bacteria on many different plates and pick every resistant colony or (2) start many different cultures, each grown from a single colony of sensitive bacteria, spread one plate from each culture, and then pick a single mutant from each plate. which method would ensure that you are isolating many independent mutations?

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Suppose you wanted to study genes controlling the structure of bacterial cell surfaces. you decide t...

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