Suppose that 1 mL of an enzyme solution can completely catalyze 10 mL of substrate in 8 minutes. Now suppose that you change the amount of enzyme, the amount of substrate, or add another substance to the mixture. How would each type of change affect the reaction rate? Match the results you would expect with each change to the experimental design. The same result may occur with more than one experimental change.
1. Use 0.5 mL of enzyme instead of 1 mL
2. Use 5 mL of substrate mixed with 5 mL water instead of 10 mL of substrate
3. Add a molecule to the mixture that preferentially binds and blocks the enzyme's active site
4. Use 20 mL of substrate instead of 10 mL
a. The reaction rate will be close to zero over the entire 8 minutes
b. The reaction rate will remain steady over the entire 8 minutes
c. The reaction rate will approach zero before 8 minutes.