QUESTION 2 (ENZYME ACTIVITY] The investigation was carried out to determine the effect of a detergent containing fat-
digesting enzymes on a fat stain.
The procedure was as follows:
Two identical cloths were used.
Cloths A and B had identical fat stains.
Cloth A was washed using a detergent not containing fat-digesting enzymes for five
minutes at 37°C.
Cloth B was washed using a detergent containing fat-digesting enzymes for five minutes
at 37°C.
The diagram below shows the results of the investigation.
Cloth B
Cloth A
Fat stain
(2)
2.1
Which property of enzymes is being investigates?
(2)
2.2
State TWO factors that were kept constant during this investigation.
(1)
2.3
What was the purpose of cloth A in the investigation?
2.4
State what should have happened to the enzymes if cloth B was washed in boiling
(1)
water.
(2)
2.5
Why do most washing powders contain protease as their enzyme?
(2
2.6 State a conclusion for this investigation.
(10)​

Punnett squares are used to show possible combinations of alleles or to predict the probability of a trait occurring in offspring. an incomplete dominance cross is performed between a bird that is homozygous for red feathers and a bird that is homozygous for blue feathers. purple offspring result. then, two of the purple offspring are crossed. according to the punnett square for this cross, how many of the offspring from the second cross will have a feather color that results from incomplete dominance? 1 in 4 2 in 4 3 in 4 4 in 4

Astudent from one of the research labs is having trouble preparing a slide for examination and photographing. the bacterial slide that he has brought to you was prepared using a commercially purchased stain. he has asked for your in determining what he is doing wrong so that he can change the lab protocols and continue on with his project. after examining the slide under oil immersion, you determine that no bacteria are present even though the student is able to show you the culture he used to make that slide that has visible growth in the liquid medium. which of the following statements does not explain the fact that there are no bacteria present on the student’s slide? by not allowing a glass slide to completely air dry before heat fixation, the flame will cause the surrounding water to boil and this will damage the bacterial cell. overheating during the fixation step boiled the water within the bacterial cells and resulted in the cells bursting. insufficient heating of the slide did not drive out the thin layer of water and this resulted in minimal bonding between the bacteria and the glass slide. rinsing with alcohol during the washing step stripped the bacteria off the glass slide. rinsing with alcohol during the washing step stripped the bacteria off the glass slide.

An enzymatic hydrolysis of fructose-1-p, fructose-1-p(aq) + h2o(l) - fructose (aq) + pi (aq) was allowed to proceed to equilibrium at 25°c. the original concentration of fructose-1-p was 0.2 m, but when the system had reached equilibrium, the concentration of fructose-1-p was only 6.52 x 10^-5 m. calculate the equilibrium constant for this reaction and the free energy of hydrolysis of fructose-1-p.