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Biology, 12.03.2020 21:30 Asrys

5. Here is another promoter region in E. coli. (Also in a larger format in the BlackBoard file) This one is between two genes. The gene whose start codon is underlined in red encodes an amino acid / proton symport. The gene whose start codon is underlined in blue encodes a protein used in glycolysis. The binding site for the CAP protein (with its cAMP cofactor) is indicated in green. A. Both of these genes are transcribed by ļ³ 70, whose "best consensus" binding site is in your Lecture 14 slides. Each promoterā€™s -35 site is indicated with a box. Copy-paste the sequence to your answer paper. Find and put a red/blue box around each promoterā€™s -10 sequence. Which is the "poorer" promoter ā€“ the red or the blue? What are two consequences of a gene having a poor promoter?

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5. Here is another promoter region in E. coli. (Also in a larger format in the BlackBoard file) This...
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