In a controlled experiment, what is manipulated by the researcher?

In a controlled experiment, an independent variable (the cause) is systematically manipulated and the dependent variable (the effect) is measured; any extraneous variables are controlled. The researcher can operationalize (i.e. define) the variables being studied so they can be objectivity measured.

many other transport proteins, often called pumps, move materials against a concentration gradient. active transport drives molecules across a membrane from a region of lower concentration to a region of higher concentration. this process, shown in figure 5.1, uses transport proteins powered by chemi- cal energy.