You have isolated the mRNA for the human insulin gene and reverse transcribed it into cDNA. Explain how you could use this cDNA to make a recombinant plasmid and bacteria to commercially produce insulin to be used as a therapeutic drug. Include all of the following terms in your answer (draw a diagram!):
Insulin gene (cDNA)
Plasmid
Host Cell
Recombinant Plasmid
Selective Media containing antibiotic
DNA Ligase
Sticky Ends
Restriction Enzyme
Sticky Ends Hybridize
Transform(ation)
Selection Using Antibiotic Resistance Gene
Lac Z gene

What part of the menstrual cycle is the femal most likely to get pregnant and why

A10-year-old girl suffered from a rare malignancy in her brain and around her spinal cord. she had surgery, and most of the tumor mass was removed, but residual tumor remained in the brain and around the spinal cord. the girl's doctors informed her parents that chemotherapy and radiation were possible treatment options but could cause serious problems such as sepsis, a permanent loss of iq and stature, and even death. the parents wished to proceed with the therapy. while their child was undergoing aggressive chemotherapy and radiation, the parents did independent research and read of several drugs being administered for cancer in other states that were unapproved by the federal drug administration (fda) and were illegal in their home state but were touted by physicians using them as miracle cures." furthermore, one of the girl's doctors released the girl's medical records to another physician to get a "second opinion" without informing the parents. this particular physician is a member of the same country club as the parents. he happens to share facts from the medical records with other members who end up telling the girl's parents. the couple sued their child's physician for failure to disclose alternative treatments, thus depriving them of informed consent. the couples also sued the child's physician for sharing their daughter's records in violation of the girl's privacy. how should the court rule in both cases? did the child's physician involved follow the law? did he act ethically in either situation? explain your answer. under the doctrine of informed consent, should a physician be responsible for informing patients of all treatment options, even if some of the treatments are illegal or not yet proven effective?

You are caring for a patient with an anxiety disorder on the psychiatric unit. the family is present with the patient. your instructor talked in the mental health course about cognitive, affective, and behavioral psychiatric nursing interventions. a. what are cognitive, affective, and behavioral psychiatric nursing interventions? b. give some examples of cognitive, affective, and behavioral psychiatric nursing interventions you could apply to the care of this family.