1) from the residue weight of the amino acids determine the amino acid composition (number of amino acids) and the minimum molecular weight of the protein. (hint best to set this up on an excel spread sheet). 2) from the calculated minimal molecular weight calculate the number of moles in 5 ml of a 150 mg/ml solution of this protein? 3) what is the molar concentration of this solution? although the last two questions seem a bit trivial, the size of the protein puts your normal frame of reference for concentrations several orders of magnitude smaller.

amino acid unk std

ala

5268

1498

arg

3130

2002

asn

4230

1804

asp

3812

1393

cys

1575

2015

gln

2902

1856

glu

4107

2102

gly

6309

1614

his

1321

676

ile

4150

1517

leu

4420

1414

lys

3807

1948

met

1712

1460

phe

3341

1710

pro

785

335

ser

4737

1515

thr

2775

1775

trp

790

2021

tyr

4525

1654

val

4950

1583