The emancipation prolcocamaion free slaves from which nation

I believe it affected all of the states, but it had the biggest impact on the southern states (confederate states).

Explanation: "all persons held as slaves within any States, or designated part of the State, the people whereof shall be in rebellion against the United States, shall be then, thenceforward, and forever free."

I apologize if this wasn't exactly what you needed!

answer; i believe the two groups were irish immigrants and chinese immigrants;