1. [Insert the was MOST impactful change on the Native Americans here and explain] a.
2. [Insert the next impactful change here and explain)
3. [Insert the next impactful change here and explain]
4. [Insert the next impactful change here and explain]
5. [Insert the next impactful change here and explain]

Explanation:1-infectious Eurasian diseases  

2-attempts of assimilation (and Manifest Destiny)  

3-relocation attempt (Indian Removal Act of 1830)  

4-diminished food supply for nomadic tribes  

5-the introduction of horses and other animals to the new world (through the Columbian Exchange)

treaties

explanation:

this isn't a question and if you're going to list things be kind and use commas.