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Health, 25.11.2021 17:30 isabelsmhl

In the "sandwich" elisa technique, what is the function of the capture antibody?

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Poliovirus is an rna virus of the picornavirus group, which uses its rna as mrna. at its 5' end, the rna genome has a viral protein (vpg) instead of a cap. this is followed by anontranslated leader sequence, and then a single long protein-coding region (~7000 nucleotides), followed by a poly-a tail. observations were made that used radioactive amino acid analogues. short period use of the radioactive amino acids result in labeling of only very long proteins, while longer periods of labeling result in several different short polypeptides. what conclusion is most consistent with the results of the radioactive labeling experiment? a) host cell ribosomes only translate the viral code into short polypeptides.b) the rna is only translated into a single long polypeptide, which is then cleaved into shorter ones.c) the rna is translated into short polypeptides, which are subsequently assembled into large ones.d) the large radioactive polypeptides are coded by the host, whereas the short ones are coded for by the virus.
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In the "sandwich" elisa technique, what is the function of the capture antibody?...
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