Your review involves analysis of data regarding the effectiveness of the vaccine in clinical trials. one method used to assess vaccine efficacy is indirect elisa. arrange the following steps to describe the sequence of events in performing an indirect elisa.
(a) an anti-antibody is added to the wells
(b) the patient serum is added to the wells
(c) upon addition of substrate, the well changes color.
(d) viral antigen-specific antibodies bind to antigen in the wells.
(e) an antigen is attached to wells in a plates.
(f) the uncoated surfaces of the plate are blocked.