Why should there be only one variable in an experiment

the answer is the last one

the symbol of the grandfather's heritage in this excerpt from "the medicine bag,” is the beaded bolo tie.

a bolo tie is a cord fastened around the neck with an ornamental clasp which is worn as a necktie. they have been used by native american tribes such as hopi, navajo, zuni, and puebloans since the mid-20th century.

therefore, the fact that the grandfather is wearing a bolo tie refers to his heritage as native american.