Jalyn would like to add some transition words signaling that Anh lined up after the competition. Which transition phrase BEST shows the sequence of events in this passage? "Anh lined up on the stage with the rest of the contestants."

Answer choices for the above question
A. At the end of the competition, Anh lined up on the stage with the rest of the contestants.
B. Before Anh lined up on the stage with the rest of the contestants, she went backstage.
C. In the community center, Anh lined up on the stage with the rest of the contestants.
D. Anh lined up on the stage, in front of the curtain, with the rest of the contestants.

his study aimed to evaluate the possibility of cotton waste enrichment with glycine betaine (GB) for production of two strains (P9, P10) of king oyster (Pleurotus eryngii). Cotton waste was used as (100%) control (T0 = cotton waste) and augmented with various combinations of GB, (T1 = 2 mmol L−1, T2 = 4 mmol L−1, T3 = 6 mmol L−1, T4 = 8 mmol L−1 and T5 = 10 mmol L−1). The response of king oyster to GB was evaluated by earliness, yield, biological efficiency (BE), minerals (nitrogen, phosphorus, potassium, zinc (Zn), copper (Cu), magnesium (Mg), manganese (Mn), iron (Fe), sodium (Na), calcium (Ca)), total sugars, total soluble solids, reducing sugars, non‐reducing sugars, ascorbic acid, proximate (crude protein, carbohydrates, crude fibers, ash, fats) content of fruiting body and Fourier‐transform infrared (FTIR) spectroscopy analysis compared with the control substrate (cotton waste).

RESULTS

The earliness, yield, and BE were higher as compared to control substrate and increased with an augmentation in the concentration of GB within the cotton waste. Two strains showed (on dry weight basis) 33.9–54.9 mg g−1 nitrogen, 6.8–12.5 mg g−1 phosphorus, 16.9–25.1 mg g−1 potassium, 40.5–64.2 mg kg−1 Zn, 17.1–37.3 mg kg−1 Cu, 1174–1325 mg kg−1 Mg, 20.1–29.1 mg kg−1 Mn, 129–265 mg kg−1 Fe, 779–835 mg kg−1 Ca), 6.3%–11.3% total sugars, 7.3–14.9 °Brix total soluble solids, 2.1–7.3% reducing sugars, 10.4–18.1% crude protein, 3.6–4.4% crude fiber and 5.6–16.7 mg (100 g)‐1 on various concentration of GB enrich cotton waste. Cotton waste enriched with GB significantly affected nutritional profile of king oyster mushroom.

CONCLUSION

The results revealed that GB enriched cotton waste can be used as an innovative substrate to enhance the yield and quality of king oyster mushroom.

Explanation:

the answer is slaves

douglas was a slave who turned into a statesman and he refused to celebrate the 4th of july until all slaves were emancipated or set free.

yes this is an analog in my view