Engineering, 23.05.2020 18:57 liyahguyton

In isolation, a DNA-binding protein binds to its regulatory sequence with a Kd of 1.0 μM. Another DNA binding protein binds to another sequence on the same DNA a few bases away with a Kd of 5.0 μM when alone. The two proteins each have a domain which binds to the other, with an interaction energy of -2.7 kcal/mole:
(a) Draw the thermodynamic box which represents all four states of this system.
(b) what are the affinities for each protein in the presence of saturating amounts of the other?

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In isolation, a DNA-binding protein binds to its regulatory sequence with a Kd of 1.0 μM. Another DN...

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