Substances A and B have retention times of 16.63 and 17.63 min, respectively, on a 30 cm column. An unretained species passes through the column in 1.30 min. The peak widths (at base) for A and B are 1.11 and 1.21 min, respectively. Calculate the time in minutes required to elute the two species with resolution Rs

The time required to elute the two species is 53.3727 min

Explanation:

Given data:

tA = retention time of A=16.63 min

tB=retention time of B=17.63 min

WA=peak of A=1.11 min

WB=peak of B=1.21 min

The mathematical expression for the resolution is:

The mathematical expression for the time to elute the two species is:

Here

ReB = 1.5