8. (4pts) Based upon your estimated purity of the E3 fraction in the question above, and upon knowing the amount of protein present in your fraction, estimate the total yield (in ug) of rGFP obtained using this protocol. You must show/explain how you calculated your answer.

An ideal otto cycle with air as the working fluid has a compression ratio of 8. the minimum and maximum temperatures in the cycle are 300 k and 1340 k. use constant specific heats at room temperature to determine (a) the amount of heat transferred to the air during the heat- addition kj/kg, (b) the thermal efficiency, and (c) the thermal efficiency of a carnot cycle ope limits. process, in rating between the same temperature