8. (4pts) Based upon your estimated purity of the E3 fraction in the question above, and upon knowing the amount of protein present in your fraction, estimate the total yield (in ug) of rGFP obtained using this protocol. You must show/explain how you calculated your answer.

Refrigerant 134a enters an insulated compressor operating at steady state as saturated vapor at -26°c with a volumetric flow rate of 0.18 m3/s. refrigerant exits at 9 bar, 70°c. changes in kinetic and potential energy from inlet to exit can be ignored. determine the volumetric flow rate at the exit, in m3/s, and the compressor power, in kw.

Aflywheel accelerates for 5 seconds at 2 rad/s2 from a speed of 20 rpm. determine the total number of revolutions of the flywheel during the period of its acceleration. a.5.65 b.8.43 c. 723 d.6.86

Ajournal bearing has a journal diameter of 3.250 in with a unilateral tolerance of 20.003 in. the bushing bore has a diameter of 3.256 in and a unilateral tolerance of 0.004 in. the bushing is 2.8 in long and supports a 700-lbf load. the journal speed is 900 rev/min. find the minimum oil film thickness and the maximum film pressure for both sae 20 and sae 20w-30 lubricants, for the tightest assembly if the operating film temperature is 160°f. a computer code is appropriate for solving this problem.