The steps of protein immunoblotting (commonly called western blotting) are listed. Step 1: Perform SDS–PAGE on protein samples. Step 2: Transfer proteins from gel to membrane Step 3: Probe membrane with labeled antibodies. Select the statement that best describes the purpose of step 1.

An engine, weighing 3000 n, is supported on a pedestal mount. it has been observed that the engine induces vibration into the surrounding area through its pedestal at the maximum operating speed. determine the stiffness of the dynamic vibration absorber spring in (n/m) that will reduce the vibration when mounted on the pedestal. the magnitude of the exciting force is 250 n, and the amplitude of motion of the auxiliary mass is to be limited to 2 mm note: in this question type-in right numbers, no decimals, no fractions, no unit. approximate to right number if needed