Chemistry, 22.11.2021 23:10 paulawells11

Thin-layer chromatography explain

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Answer from: dianaosorio33895

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Thin layer chromatography explained?

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Thin layer chromatography (TLC) is an affinity-based method used to separate compounds in a mixture. TLC is a highly versatile separation method that is widely used for both qualitative and quantitative sample analysis. TLC can be used to analyze virtually any substance class, including pesticides, steroids, alkaloids, lipids, nucleotides, glycosides, carbohydrates, and fatty acids.

In TLC, the stationary phase is a thin adsorbent material layer, usually silica gel or aluminum oxide, coated onto an inert plate surface, typically glass, plastic, or aluminum. The sample is spotted onto one end of the TLC plate and placed vertically into a closed chamber with an organic solvent (mobile phase). The mobile phase travels up the plate by capillary forces and sample components migrate varying distances based on their differential affinities for the stationary and mobile phases. When the solvent reaches the top of the plate, the plate is removed from the developing chamber and dried. The separated components appear as spots on the plate and the retention factor (Rf) of each component is assessed.

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Answer from: Quest

by cartels in other countries, shipped by air, mail, or land. i cant really see your answer choices : /

Answer from: Quest

For cyclohexane compounds drawn in a chair conformation: -axial bonds are vertical -equatorial bonds are parallel to two bonds within the ring. thus, as i assume the chair conformation is drawn: -the x group is in an axial position, as are the five other vertical bonds. -three axial bonds are on the top face of the ring -the other three axial bonds are on the bottom face the position of the second substituent on each ring carbon is equatorial. gauche groups must be on an adjacent carbon such as carbon 6 and carbon 2, and also gauche groups are rotated by 60 degrees relative to each other. drawing the newman projections for c1–c2 and c1–c6 aids in visualizing the groups that are gauche to the y group on c1. click this link and watch some academic videos that may you more understand the topic

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