To run a thin layer chromatography experiment with a chemical substance, begin by marking a horizontal line near the bottom of a TLC plate with . Place a spot of the substance onto the line. For the mobile phase, add a small amount of at the bottom of a TLC chamber. Place the plate in, then the chamber. Once the mobile phase approaches the top of the plate, remove the plate and mark the line. Note the position of the spots and calculate Rf values if needed. A. Options for blank 1: pen, pencil
B. Option for blank 2: large, small
C. Options for blank 3: solvent, solid
D. Options for blank 4: cover, shake
E. Options for blank 5: starting, solvent