Acetoacetyl-ACP is formed as an intermediate during fatty acid biosynthesis. The CO2 used to synthesize malonyl-CoA is lost. Would this help make the reaction more or less energetically favorable

This would make the reaction more energetically favourable

Explanation:

Fatty acid synthesis starts with the carboxylation of acetyl CoA to malonyl CoA. This irreversible reaction is the committed step in fatty acid synthesis. The synthesis of malonyl CoA is catalyzed by acetyl CoA carboxylase, which contains a biotin prosthetic group.