Given 200ul of a 0.5mg/ml stock solution of BSA, how much do you pipet into a test tube so that you are adding 5ug of BSA to the test tube

of volume needs to be pipetted out in the test tube.

Explanation:

We are given:

Mass of BSA to be formed =      (Conversion factor:

Volume of stock solution =    (Conversion factor:

It is also given that for the mass of BSA is 0.5 g, the volume used up is 1 mL

In order to have, 0.005 g, the volume of stock solution needed will be =

Hence, of volume needs to be pipetted out in the test tube.