1a - Design primers to clone your gene Phenylalanine hydroxylase into pET28a plasmid. 1b -. Explain your reason behind designing the specific primers: Restriction sites, presence of tags.

alchemy hadn't scientific methods of study, appeal to mysticism and fantasy, the knowledge of chemistry and physics were modest at that time.

alchemy was - but as new rules were discovered it either changed into chemistry or it became "unscientific".