Visualizing spots once the plate is run is crucial in determining the components in the mixture. The above TLC was stained with vanillin in sulfuric acid to visualize the compounds in each essential oil. In our lab, UV will visualize compounds with conjugated double bonds and iodine will stain double bond-containing compounds and some oxygen containing functional groups. Which visualization method would you perform on each of the five molecules above

Answer asap need by wednesday morning explain how a buffer works, using an ethanoic acid/sodium ethanoate system including how the system resists changes in ph upon addition of a small amount of base and upon addition of a small amount of acid respectively. include the following calculations in your i. calculate the ph of a solution made by mixing 25cm3 0.1m ch3cooh and 40cm3 0.1m ch3coo-na+. [ka = 1.74 x 10-5 m] ii. calculate the ph following the addition of a 10cm3 portion of 0.08 m naoh to 500cm3 of this buffer solution. iii. calculate the ph following the addition of a 10cm3 portion of 0.08 m hcl to 200cm3 of the original buffer solution.