Determine the amino acid sequence of a polypeptide from the following data: Complete hydrolysis of the peptide yields Arg, 2 Gly, Ile, 3 Leu, 2 Lys, 2 Met, 2 Phe, Pro, Ser, 2 Tyr, and Val. Treatment with Edman's reagent releases PTH-Gly. Carboxypeptidase A releases Phe. Treatment with cyanogen bromide yields the following three peptides: 1. Gly-Leu-Tyr-Phe-Lys-Ser-Met
2. Gly-Leu-Tyr-Lys-Val-Ile-Arg-Met
3. Leu-Pro-Phe
Treatment with trypsin yields the following four peptides:
1. Gly-Leu-Tyr-Phe-Lys
2. Ser-Met-Gly-Leu-Tyr-Lys
3. Val-Ile-Arg
4. Met-Leu-Pro-Phe
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