1. We will now look at a little bit of biochemistry as we explore the idea of median lethal doses of chemical consumption.
A. Using the concentration of Allura red you found in the original, undiluted sample of cherry Kool-Aid, as well as the LD50 of Allura red given in the lab write-up, determine the volume (in liters) of Kool-Aid you must ingest to reach toxic levels of red food coloring. Assume the LD50 for humans is the same as that for mice. Show all calculations below.
B. if you then assume that the volume of Kool-Aid is virtually all water, what volume of Kool-Aid (in liters) would you need to ingest in order to reach toxic levels of water? (HINT: The density of water is 1.00 g/mL).
C. Given your answers to parts a and b, summarize your findings as you answer the question of the Day. Support your answer with specific evidence.
2. calculate the number of moles of Allura red found in 100.0 mL of the 1.89 X 10" M stock solution. What volume of your original Kool-Ald sample, do you need to have the same amount of moles of Allura red?
Concentration Allura Red Used: 1.89 x 10^-4 M
Volume Allura Red (mL) Concentration (M)* Absorbance
5.00 4.725 x 10^4 0.220
10.00 3.78 x 10^-5 -0.435
15.00 2.835 x 10^-4 0.636
20.00 1.89 x 10^-5 0.800
25.00 0.945 x 10^-5 1.004
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