Chemistry, 05.03.2021 06:00 naomicervero
Tris is a molecule that can be used to prepare buffers for biochemical experiments. It exists in two forms: Tris (a base) and TrisH (an acid). The MW of Tris base is 121.14 g/mol; the MW of TrisH is 157.6 g/mol (the extra weight is due to the Cl- counterion that is present in the acid). The Ka of the acid is 8.32 X 10-9. Assume that you have TrisH in solid form (a powder), unlimited 1M HCl, unlimited 1 M NaOH and an unlimited supply of distilled water. How would you prepare 1 L of a 0.02 M Tris Buffer, pH
Answers: 2
![$K_a = \frac{[\text{Tris}^-]\times[\text{H}_3\text{O}^-]}{[\text{TrisH}^+]}$](/tpl/images/1170/9735/4efa1.png)
moles of acid
, we must have an 
concentration of ![$[OH^-] = 10^{-pOH}$](/tpl/images/1170/9735/e3cb0.png)
moles of base
moles
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Tris is a molecule that can be used to prepare buffers for biochemical experiments. It exists in two...
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