A student does a PCR to make copies of the HDR template transformation. They column-clean the PCR, eluting in 20 ul of warm elution buffer. They use 2 ul of the 20 ul stock to nanodrop the sample, and determine the concentration is 60 ng/ul. How many nanograms of HDR template does the student have left to use for future work (e. g. yeast transformations)

the student will have 1080 nanograms of HDR template left to use for future work

Explanation:

Given that:

the total volume of DNA which is eluted after the PCR clean up = 20ul

PCR clean up is a procedure by which the PCR product is gotten rid of DNA polymerase enzyme, excess primers, excess dNTPs so that these does not interfere in further experiments such as transformation.

using 2 ul of the 20 ul stock to nanodrop the sample

Concentration of the DNA after the PCR clean up = 60ng/ul

No, Since 2ul is used in the nanodrop, thus total volume left for further experiment will be = 20 - 2 = 18ul

Now, the total amount of the HDR template the student have left to use for future work will be;

⇒ 18ul × 60 ng/ul

= 1080 ng

Therefore, the student will have 1080 nanograms of HDR template left to use for future work

just did the assignment and the answer was #1 +2 n

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