A mixture of enzyme and inhibitor is run through a size-exclusion chromatography column. The activity of the enzyme is assessed before and after the chromatography. The enzyme has more activity after the chromatography step. Which of the following is true? a. The inhibitor is an irreversible inhibitor.
b. The enzyme was denatured during chromatography.
c. The enzyme was not eluted fully from the column.
d. The enzyme was degraded by the column.
e. The inhibitor is a reversible inhibitor.