Perform a DNA extraction from green peas at home or school lab, under adult supervision, by following the given procedure carefully.
Procedure for DNA extraction:
To half cup of split peas, add 1/8 teaspoon of salt and one cup of cold water.
Transfer this mixture in a blender and blend it on high speed for 15 seconds.
Filter the blended mixture, using a strainer, into a measuring container. Observe the amount of mixture you obtain after filtration. Add liquid detergent equal to 1/6 volume of the obtained pea mixture.
Mix by swirling. Allow the mixture to rest for about five to ten minutes.
Transfer this mixture to test tubes. Fill the tubes to about 1/3 of their volume.
Add a pinch of meat tenderizer to each of these tubes. Mix gently to avoid damaging the DNA.
Tilt the test tube, and pour alcohol (75% isopropyl alcohol or 95% ethanol) down the sides of the tube. Add alcohol to about 2/3 volume of the tube. You will obtain two layers. The layer at the top contains the DNA.
Now that you are done with the DNA extraction process, answer the following questions:
What purpose does blending the pea mixture serve?
Why do you need to add liquid detergent?
What role does the meat tenderizer play?
Why is alcohol added and why does the DNA get extracted in the alcohol layer?
Answers: 3
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