You have two compounds that you have spotted on the TLC plate. One compound is more polar than the other. You ran the TLC plate with neat hexanes and found that only one spot moved a small distance up the plate. What might you expect to see if you ran a TLC plate in a 50/50 mixture of hexanes and ethyl acetate

we will except an increase in the polarity of the system and this will cause the Non-polar spot to be near the solvent front, while the polar spot will run at an approximate speed of 0.5 Rf

Explanation:

when we run a TLC plate in a 50/50 mixture of hexanes and ethyl acetate we will except an increase in the polarity of the system and this will cause the Non-polar spot to be near the solvent front, while the polar spot will run at an approximate speed of 0.5 Rf

The speed of the polar spot depends largely on the level of polarity, an increase in the polarity will see both spots of Neat hexane run when we run a TLC  plate in a 50/50 mixture of hexanes and ethyl acetate

the old alchemists made significant contributions to science even though there starting premises were off a bit. their conclusions after failure would steer them into better directions for investigation. science is no more than close observation and the initial mistakes result in rethinking and retrying experiments.