Fructose‑2,6‑bisphosphate is a regulator of both glycolysis and gluconeogenesis for the phosphofructokinase reaction of glycolysis and the fructose‑1,6‑bisphosphatase reaction of gluconeogenesis. In turn, the concentration of fructose‑2,6‑bisphosphate is regulated by many hormones, second messengers, and enzymes.