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Chemistry, 22.06.2019 05:30
A3.37-mg sample of protein was chemically digested to convert its nitrogen into ammonia and then diluted to 100.0 ml. then 10.0 ml of this solution was placed in a 50-ml volumetric flask and treated with 5 ml of phenol solution plus 2 ml of sodium hypochlorite solution. the sample was diluted to 50.0 ml, and the absorbance at 625 nm was measured in a 1.00-cm cuvette and found to be 0.486. for reference, a standard solution was prepared from 10.0 mg of nh4cl (molar mass = 53.49 grams/mole) dissolved in 1.00 l of water. then 10.0 ml of this standard was placed in a 50-ml volumetric flask, treated in the same manner as the unknown, and the absorbance found to be 0.323. finally, a reagent blank was prepared using distilled water in place of unknown, it was treated in the same manner as the unknown, and the absorbance found to be 0.076. calculate the weight percent of nitrogen in the protein.
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Chemistry, 22.06.2019 21:00
Need what is special about water as a compound? how does water regulate climate? what drives water evaporation? why is the water vapor fresh water when it rises from the ocean? why might freshwater in the form of snow take longer to enter the water cycle again than liquid precipitation? what is an aquifer? what role do people play in the water cycle? plz just answer as many as you can ! thx if you !
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