Experiments performed with biological systems require a buffer that will not interfere with the stability and activities of the biological components. In 1966, Norman Good and his colleagues began a series of investigations that established criteria for new zwitterionic buffers that would be optimal for experimentation with biological systems. These criteria included: a. pKa between 6 and 8
b. highly water soluble
c. minimally permeable to biological membranes
d. minimal effects of salt concentration
e. minimal effect of temperature on pKa (pKa/°C)
f. minimal effect of concentration on pKa minimal interaction with metal ions non-toxic
g. high chemical stabilit
h. minimal absorbance between 240-700 nm
For each buffer, choose the property that does not meet Good's criteria for biochemical buffers If all of the buffer's properties satisfy the criteria for a Good's buffer:
Properties of acetate buffer: Properties of MOPS buffer:
1. highly water soluble pKa at 25°C 7.15
2. ApKJ°C=-0.0002 ΔpKJ°C =-0.013
3. pKg at 25°C = 4.76 absorbs light at <230 nm
4. does not cross lipid bilayer does not cross lipid bilayer
5. all the above properties fulfill all the above properties fulfil
the lipid criteria the lipid criteria

if the concentration of products is decreased, the equilibrium will shift toward the products). add an inert gas (one that is not involved in the reaction) to the constant-volume reaction mixture: this will increase the total pressure of the system, but will have no effect on the equilibrium condition.

explanation:

when—without warning—something provokes your anger, you may struggle not to succumb to it. since what typically makes you mad is feeling powerless in the face of what seems unfair, your anger is mostly an attempt at a “quick fix” to right the balance. it’s as though you’re raising a fist in protest, proclaiming that you’re not going to capitulate to such injustice.

there are, however, a multitude of problems related to this immediate, push-back reaction of anger. and probably the key one is that almost never does it resolve the issue that gave rise to it. such reactive anger is probably best understood as self-defeating. as david burns, the author of the seminal self- book feeling good, observes: if realistically, acting on your anger is to make any sense, it needs to meet two criteria—which, in almost every case, is frankly impossible. that is, your anger must:

be directed toward a person who has intentionally (and needlessly) behaved in a hurtful way toward you; and

be beneficial or advantageous to you (i.e., assist you in achieving a desired goal).

i think you’ll agree that only rarely can you claim that your anger is both warranted and , whether to yourself or the relationship.

so let me offer you a two-step alternative to abandoning your better judgment and giving in to the temptation of anger—one that should neutralize your anger in seconds. or, when you’re really angry, in minutes.

but keep in mind that you must really want to execute these steps, be sufficiently motivated to perform them. which means overcoming more unconscious resistance than you might ever have imagined. because there are many immediate “advantages” of anger that can interfere with your resolve, i’ll suggest a few of them that might interfere with your employing this powerful method to rid yourself of counter-productive anger.

explanation:

the answer to your question is c.

if you read the house of usher you should know that the house was haunted

it would be b. 6co2 + 6h2o --> c6h12o6 + 6o2