subject
Chemistry, 06.05.2020 04:45 pstat

Move the filled cuvette containing the 100 µM solution to the spectrophotometer and load into the sample chamber as before. With the spectrophotometer in the Full Spectrum Mode, obtain a spectrum of the EDMAB solution, and click the REC button to display it below. You might need to scroll this text area to see the chart. Refer to the note you made in your lab notebook about the wavelength of maximum absorption for EDMAB in ethanol. Pay particular attention to the sharp peak around 300 nm. Notice how much stronger this peak is than anywhere else in the range 200-600 nm. You need to locate this peak experimentally by adjusting the wavelength setting of the spectrometer. Switch the instrument back to Single Wavelength Mode. Find the absorbance maximum for the sample by entering wavelengths and reading absorbancies until you have found the maximum. There may be more than one maximum, so explore the frequency range to be certain you have found the wavelength with overall maximum absorbance. You should be able to determine the maximum value to within 1 nm. The strongest absorption peak is quite sharp, and you will need to explore carefully to find the wavelength which has the strongest absorption. This process need only be performed now at the beginning of the analysis. Hint: The correct wavelength will be slightly greater than 300 nm, and the absorbance of the sample at the maximum will be greater than 2 a. u.

ansver
Answers: 1

Another question on Chemistry

question
Chemistry, 22.06.2019 03:30
The atomic radius of sodium is 186 pm and of chlorine is 100 pm. the ionic radius for na+ is 102 pm and for cl– is 181 pm. in going from na to cl in period 3, why does the atomic radius decrease while the ionic radius increases? a. the inner electrons in the sodium cation shield its valence electrons more effectively than the inner electrons in the chloride anion do. b. the inner electrons shield the valence electrons more effectively in the chlorine atom than in the chloride anion. c. the outermost electrons in chloride experience a smaller effective nuclear charge than those in the sodium cation do. d. the outermost electrons in chloride experience a larger effective nuclear charge than those in the sodium cation do. e. monatomic ions are bigger than the atoms from which they are formed.
Answers: 2
question
Chemistry, 22.06.2019 11:30
For each of the following compounds, decide whether the compound's solubility in aqueous solution changes with ph. if the solubility does change, pick the ph at which you'd expect the highest solubility. you'll find ksp data in the aleks data tab. compounds does solubility change with ph
Answers: 3
question
Chemistry, 22.06.2019 14:30
1) describe the physical layout of the ocean floor ? 2) explain how the dumbo octopus swims differently than other octopus species and why this would be an advantage in the aphonic zone . 3) why are the types of organisms that live at each underwater hot vent so dramatically different ?
Answers: 3
question
Chemistry, 22.06.2019 17:30
98 points you will be galileo perform the experiment to determine if objects with different mass fall at the same, or different, rates in the air and in a vacuum. before you conduct your experiment, you need to form a hypothesis. a hypothesis is a prediction of what you think will happen in the experiment. the hypothesis is a statement that describes “if” a certain set of circumstances are present “then” there will be a specific result that will occur. record your hypothesis here: record the results from step one of the experiment (dropping the objects in the air): first trial: second trial: third trial: record the results from step two of the experiment (dropping the objects in a vacuum): first trial: second trial: third trial: did the experiment support your hypothesis? using the data from your experiment, describe why you believe your hypothesis was either proven or disproven. what forces were acting on the objects dropped in the air? what force was acting on the objects dropped in the vacuum? part two: comparing forces choose two forces and compare and contrast these forces. you must provide two ways that they are alike and two ways that they are different. you may make a list, write in paragraph form, or make a chart. choose two forces and compare and contrast these forces. these must be different forces than used in the prior question. provide two ways that they are similar and two ways that they are different. you may make a list, write it out, or make a chart.
Answers: 3
You know the right answer?
Move the filled cuvette containing the 100 µM solution to the spectrophotometer and load into the sa...
Questions
question
History, 08.10.2021 14:00
question
Chemistry, 08.10.2021 14:00
question
Mathematics, 08.10.2021 14:00
question
Computers and Technology, 08.10.2021 14:00
question
Mathematics, 08.10.2021 14:00
Questions on the website: 13722367