While testing amylase activity, john uses a buffer of ph 6 and substrate starch. he incubates them at 104ºf (40ºc). all the tubes show glucose formation, except one, which had no starch solution. why did this happen?
a) the optimum ph for enzyme amylase is not ph 6
b) the optimum temputure for the enzyme amylase is not 104. f (40.)
c) the substrate for amylase, starch, is not present

An experiment is conducted to see if cats preferred skim milk or 2% milk. a cup of skim milkwas put out for 5 kittens and then measured how much the kittens drank over the course of aday. following a cup of 2% milk was purout for the skittens and then masured how much thekittens drank over the course of a day. the same kittens were used and the milk was served atthe same temperature. it was discovered that the cats liked the 2% milk more than the skimmilk. what is the dependent variable in this experiment?