Citrate, the product of reaction 1 of the citric acid cycle, inhibits phosphofructokinase, which catalyzes the third reaction of glycolysis. Based on the importance of metabolic flux and the body meeting its metabolite needs, why is this important when there is an excessive buildup of citrate?

Excessive buildup of citrate informs the body that it need not break down or degrade glucose any longer for the synthesis of biosynthetic precursor molecules needed for the synthesis of fatty acids as there are enough building blocks of this molecules in circulation.

Explanation:

The regulatory control of glycolysis are mostly found in the irreversible enzyme catalying reactions of glucolysis. The reactions catalyzed by hexokinase, phosphofructokinase and private kinase are potential sites of control. Glycolysis provides for the production of ATP through glucose degradation and also building blocks for biosynthetic processes such as the synthesis of fatty acids, the availablility of these materials should therefore inhibit the action of glycolysis. High level of ATP inhibits the enzyme phoshofructokinase in the liver and hence decrease it's affinity for fructose 6 phosphate.

In addition to that, the accumulation of citrate, an intermediate in citric acid cycle inhibits this enzyme phosphofructokinase and hence stop the process of production of biosynthetic precursors since these precursors are already in abundance. Citrate performs this inhibitory effect by enhancing the inhibitory effect of excess ATP on phosphofructokinase.

So therefore, body meets it's ATP needs and biosynthetic precursor needs and once that is done, it signals for a halt in the further production of this molecules until the substances are depleted.

according to the u.s. department of agriculture, the label of "certified organic" comes with a set of highly regulated standards for environmental stewardship and resource conservation. some of these include maintenance and improvement of soil quality, prevention of ground and surface water contamination, greenhouse gas regulation, encouragement of biodiversity and conservation of water and energy (see references 1). organic practices integrate natural methods such as mulching, plant diversity, native and adapted plants, crop rotation and the introduction of beneficial insects to control pests without synthetic pesticides.

environmental implications of pesticides

synthetic pesticides ensure high crop yields. while effective in achieving this goal, pesticides contaminate soil, air, ground and surface water, as well as the plants and animals that come into contact with these contaminated sources. freshwater and marine organisms are particularly susceptible to pesticides through agricultural runoff. pesticides also reduce soil fertility (necessitating the use of synthetic fertilizers) and can travel far from the original source, contaminating water and air hundreds of miles from the location of application (see references 2).

health implications of organics

although a subject of particular debate, many recent findings show that organic food is healthier than conventionally grown food. according to the usda, an extensive european union-funded study found that organically grown foods contain more beneficial compounds, such as the vitamins and antioxidants needed to combat many diseases (see references 5). organic milk, meat and eggs are regulated to ensure they are free of hormones administered to conventional livestock and poultry.

health implications of pesticides

pesticides affect human health in three ways: through skin contact, inhalation and ingestion. acute and long-term exposure to pesticides can cause serious health problems for farmers who apply them. the majority of other people are affected by drinking contaminated water and eating contaminated marine life (see references 3). pesticides have been linked to neurological disorders, endocrine and reproductive disorders, immune system deficiencies and cancer. according to the world health organization, acute pesticide poisoning (app) is responsible for significant annual mortality, especially in developing nations (see references 4).

other important considerations

not every small farm or business owner can afford the costly certification process required to be considered "certified organic," even if the farm is not using chemicals. "transitional" farms include those that once used chemicals and are now transitioning to organic-only production. in addition, though organic farming methods offer obvious environmental benefit, an organic apple shipped from around the world creates a different set of environmental problems. locally grown food (particularly locally grown, organic food) is key in sustainable food production.