The spectrophotometric absorbance of three different solutions could be measured in this experiment to determine the concentration ratio of unbound ferrihemoglobin (Hb. Fe3+ ) to bound ferrihemoglobin-imidazole (Hb. Fe3+.Im. These solutions, designated A, A0, and A100 are important. Use these terms to mathematically define: a) [ Hb. Fe3+ ] and b) [Hb. Fe3+.Im]

The meaning f mathematical term is given below

A - This is defined as the absorbance of that solution which have all Bound hemoglobin imidazole comlex.

so according to this [Hb.Fe3+] = 0

[Hb.Fe3+.Im]= 100

concentration can be calculated by lambert beer law

A= Cl

where A = absorbance

molar absorbance coefficient

l = path length

A0 - This is defined as the absorbance of compeletely unbound hemoglobin . this is for non complex with imidazole.

[Hb.Fe3+.Im]= 0

  [Hb.Fe3+]= 100

A100 - this is defined as the absorbance of hemoglobin imidazole complex when all hemoglobin is present in bound state.

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