Consider a second experiment: Graduate students repeated the above experiment with one modification. The same protocol was used for the LB ampicillin plate, plating 100 microliters of the reaction mix directly to the LB amp plate. However, they modified the protocol for the LB only plate by diluting the transformation mix by 1/1000 before plating. They then plated 100 microliters of this 1/200 dilution on the LB only plate. When viewing the colonies (after 2 days of growth), they obtained the following results:

hydrocarbons are organic compounds that contain only hydrogens and carbons. they can be saturated (those which contain only carbon-carbon single bonds) or unsaturated (those which contain carbon-carbon double or triple bonds) in nature. hydrocarbons can undergo several reactions like substitution, elimination etc.  

on substituting one or more hydrogen atoms in hydrocarbon it results in the formation of haloalkane. as the halogen atoms are large compared to the carbon and hydrogen atoms, due to which the molecular weight increases and the bond become polar because of presence electronegative halogen atom and results in the increase in boiling point of the haloalkane.

thus, the correct option is (b) that is the boiling point of the new compound increases on substituting a hydrogen atom with a halogen in a hydrocarbon.