4.14 Oxygen requirement for growth on glycerol Klebsiella aerogenes is produced from glycerol in aerobic culture with ammonia as nitrogen source. The biomass contains 8% ash, 0.40 g biomass is produced for each g of glycerol consumed, and no major metabolic products are formed. What is the oxygen requirement for this culture in mass terms

0.37 g

Explanation:

The molecular weight for Glycerol = 92

Number of Carbon atoms in glycerol (x)   = 3

Molecular weight of  the Biomass  ( Klebsiella aerogenes )

=

=

= 26.1

From the molecular weight of the Biomass, we can deduce the Degree of reduction for the substrate(glycerol denoted as ) as follows:

= (4×1)+(1×1.73)-(2×0.43)-(3×0.24)

= 4.15

Given that the yield of the Biomass = 0.40 g

However;

C =

C =

C = 1.41 g

Now , the oxygen requirement can be calculated as:

=

=

= 2.1 g/mol

Hence, we can say that the needed oxygen = 2.1 g/mol of the substrate consumed.

Now converting it to mass terms; we have:

=

=

= 0.3652 g

≅ 0.37 g

∴ The oxygen requirement for this culture in mass terms = 0.37 g

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