Using a spectrophotometer, and a cuvette with a path length of 1 cm you measure the absorbance (A275) of Guanosine to be 0.70. Calculate the concentration of guanosine in your sample

Answer : The concentration of guanosine in your sample is,

Explanation :

Using Beer-Lambert's law :

where,

A = absorbance of solution  = 0.70

C = concentration of solution = ?

l = path length = 1.00 cm

= molar absorptivity coefficient guanosine  =

Now put all the given values in the above formula, we get:

Thus, the concentration of guanosine in your sample is,

it have destructive character