A solute with a retention time of 407 s has a width at the base of 13.0s on a column 12.2 m long. Find the number of plates and plate height.

Answer : The number of plates and plate height is, plates and respectively.

Explanation : Given,

Retention time = 407 s

Width of a base = 13.0 s

Length = 12.2 m

First we have to calculate the number of plates.

Formula used :

where,

N = number of plates

= retention time

w = width of a base

Now put all the given values in the above formula, we get:

plates

Now we have to calculate the plate height.

Formula used :

where,

H = height of plate

L = length

N = number of plates

Now put all the given values in the above formula, we get:

Thus, the number of plates and plate height is, plates and respectively.