(c) polar solutes were separated by hydrophilic interaction chromatography (hilic) with a strongly polar bonded phase. how would retention times be affected if eluent were changed from 80 vol% to 90 vol% acetonitrile in water?

(d) polar solutes were separated by normal-phase chromatography on bare silica using methyl '-butyl ether and 2-propanol solvent. how would retention times be affected if eluent were changed from 40 vol% to 60 vol% 2-propanol? (hint: see la..12.l. g-2.i.: a.)

(c) The retention time would be higher (d) The retention time would be lower.

Explanation:

For the polar solutes which were separated using the hydrophilic interaction chromatography (HILIC) with a strongly polar bonded phase, the retention time would be higher if eluent were changed from 80 vol% to 90 vol% acetonitrile in water.

However, for the polar solutes which were separated using the normal-phase chromatography on bare silica with methyl t=butyl ether and 2-propanol solvent, the retention time would be lower if the eluent were changed from 40 vol% to 60 vol% 2-propanol.

where's the diagram?

the formula for the conversion of temperature from degree celsius to kelvin is:

the sample of water at is converted to kelvin temperature by using the above formula as:

on comparing the two samples of water at or we can say that at and at respectively. the difference between the temperatures of two samples of water is:

hence, one can say that the sample of water at is greater than the sample of water at by .