Chemistry, 13.12.2019 02:31 beebeck6573
An enzyme is found that catalyzes the reaction x ⇌ y. researchers find that the km for the substrate x is 4 μm, and the kcat is 20 min−1.
(a) in an experiment, [x] = 6 mm, and v0 = 480 nm min−1. what was the [et] used in the experiment?
(b) in another experiment, [et] = 0.5 μm, and the measured v0 = 5 μm min−1. what was the [x] used in the experiment?
(c) the compound z is found to be a very strong competitive inhibitor of the enzyme, with an α of 10. in an experiment with the same [et] as in (a), but a different [x], an amount of z is added that reduces v0 to 240 nm min−1. what is the [x] in this experiment?
(d) based on the kinetic parameters given above, has this enzyme evolved to achieve catalytic perfection? explain your answer briefly, using the kinetic parameter(s) that define catalytic perfection.
Answers: 3
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An enzyme is found that catalyzes the reaction x ⇌ y. researchers find that the km for the substrate...
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