Many enzymes are inhibited irreversibly by heavy metal ions such as hg2+ , cu2+ , or ag+ , which can react with essential sulfhydryl groups to form mercaptides. enz−sh+ag+⟶enz−s−ag+h+ the affinity of ag+ for sulfhydryl groups is so great that ag+ can be used to titrate −sh groups quantitatively. to 10.0 ml of a solution containing 1.3 mg/ml of a pure enzyme, an investigator added just enough agno3 to completely inactivate the enzyme. a total of 0.436 μmol agno3 was required. calculate the minimum molecular weight ) of the enzyme.

The minimum molecular weight of the enzyme is 29.82 g/mol

Explanation:

Step 1: Given data

The volume of the solution = 10 ml = 10*10^-3L

Molarity of the solution = 1.3 mg/ml

moles of AgNO3 added = 0.436 µmol = 0.436 * 10^-3 mmol

Step 2: Calculate the mass

Density = mass/ volume

1.3mg/mL = mass/ 10.0 mL

mass = 1.3mg/mL *10.0 mL = 13mg

Step 3: Calculate minimum molecular weight

Molecular weight = mass of the enzyme / number of moles

Molecular weight of the enzyme = 13mg/ 0.436 * 10^-3 mmol

Molecular weight = 29.82 g/mole

The minimum molecular weight of the enzyme is 29.82 g/mol

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