You perform a ligation with ncoi/noti-digested, pet-41a vector and the egfp insert from pegfp-n1 (as in lab). you then transform the ligation mix into e. coli and plate on lb medium containing kanamycin. consider the following unexpected outcomes, and suggest controls: nothing grows. what controls might you design to determine whether the e. coli cells are viable (alive) versus whether the e. coli is competent? what would the results of those controls be? you see lots of growth, but when you isolate plasmid from numerous e. coli colonies, all you find is pet-41a with no insert. suggest the most likely way these colonies arose (aside from contamination). what would the results of your controls look like if your suspicion was correct?

The density of glycerin is 1.26grams/centimeter cubed . how many is this? use the conversion rates of and . express your answer to the correct number of significant figures.

It is important to wear proper protective equipment in lab even when not actively performing experiments because accidents can affect any researcher, even one not working on an experiment. select the best answer from the choices provided

Which explanation is true about what happens to a ray of light when it strikes a mirror? a. a light ray is transmitted toward a mirror at a certain angle. the light ray is then reflected by the mirror at an equal angle but in the opposite direction of the transmitted ray. b. an incident ray strikes a mirror at an angle with a line perpendicular to the mirror. the light ray is then reflected at an angle equal to the angle of incidence but on the opposite side of the perpendicular line. c. a reflecting ray strikes a mirror at an angle with a line perpendicular to the mirror. the light ray is then refracted at an angle equal to the angle of the reflecting ray and on the same side of the perpendicular line. d. an incident ray strikes a mirror at an angle with a line parallel to the mirror. the light ray is then transmitted at an angle equal to the angle of incidence but on the opposite side of the parallel line. you so much! : -d take the time to try and answer correctly.