A300-gallon anaerobic digester will be loaded daily with a feedstock that contains two parts dairy manure and one-part water by volume. the feedstock contains 6% volatile solids (vs) by weight and has a density of 37.5 lb/ft'. what volume of feedstock would be required each day to maintain an organic loading rate (olr) of 2.0 kg vs/m/day? what is the hydraulic retention time (hrt) in the anaerobic digester tank for a loading rate of 2.0 kg vs/m/day?

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A3.37-mg sample of protein was chemically digested to convert its nitrogen into ammonia and then diluted to 100.0 ml. then 10.0 ml of this solution was placed in a 50-ml volumetric flask and treated with 5 ml of phenol solution plus 2 ml of sodium hypochlorite solution. the sample was diluted to 50.0 ml, and the absorbance at 625 nm was measured in a 1.00-cm cuvette and found to be 0.486. for reference, a standard solution was prepared from 10.0 mg of nh4cl (molar mass = 53.49 grams/mole) dissolved in 1.00 l of water. then 10.0 ml of this standard was placed in a 50-ml volumetric flask, treated in the same manner as the unknown, and the absorbance found to be 0.323. finally, a reagent blank was prepared using distilled water in place of unknown, it was treated in the same manner as the unknown, and the absorbance found to be 0.076. calculate the weight percent of nitrogen in the protein.

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