Aqueous two-phase extraction is used to purify a recombinant hivβ-galactosidase fusion peptide produced in escherichia coli. for optimum separation, 450 kg of a mixture of 19.7% w/w polyethylene glycol (peg) and 17.7% w/w potassium phosphate salt in water is needed. left over from previous pilot-plant trials is 100 kg of a mixture of 20% w/w peg in water, and 150 kg of a mixture of 20% w/w peg and 25% w/w salt in water. also on hand is 200 kg of an aqueous stock solution of 50% w/w peg, 200 kg of an aqueous stock solution of 40% w/w salt, and an unlimited supply of extra water. if all of both leftover mixtures must be used, how much of each stock solution and additional water is required?

Construct the hypothetical phase diagram for metals a and b between room temperature (20c) and 700c, given the following information: * the melting temperature of metal a is 480c. • the maximum solubility of b in a is 4 wt% b, which occurs at 420c. • the solubility of b in a at room temperature is 0 wt% b. • one eutectic occurs at 420c and 18 wt% b–82 wt% a. • a second eutectic occurs at 475c and 42 wt% b–58 wt% a. • the intermetallic compound ab exists at a composition of 30 wt% b–70 wt% a, and melts congruently at 525c.• the melting temperature of metal b is 600c. • the maximum solubility of a in b is 13 wt% a, which occurs at 475c. • the solubility of a in b at room temperature is 3 wt% a.

The skeletal system performs a variety of functions that are crucial to maintaining life processes. what function is performed in the bone marrow, but not in the ossified bones of the skeleton? a oxygen transportation c mineral storage b. muscle attachment d red blood cell production

Mrs. smith ordered a root beer float (vanilla ice cream + root beer). mrs. smith noticed that the three states of matter (solid, liquid, and gas) all existed simultaneously in her root beer float. a. identify each phase of matter in the root beer float. b. describe the particles of all three phases of matter in the root beer float. (how are the particles arranged and moving? ) c. identify one phase change you would see in a root beer float and described what causes this change.