Make a 50 ml agarose gel of 0.8% that is 0.5x tbe. the tbe (tris fw = 121.1, borate fw = 61.8, edta fw w = 372) stock is 10x. you have a 150x stock of buffer and you need 2.5 ml of 1x. how do you make this? you have a dna solution that is 21.3 mu g/mu_1 and you want 75 mu 1 that is 50 mu g/mu 1. how do you make this? make 350 ml of a solution that is 25 mm tris, 2.5 mm edta, and the ph is 8.0. tris is a base and the fw of tris is 121.1 and edta is 187. do not make a volume that exceeds 350
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Make a 50 ml agarose gel of 0.8% that is 0.5x tbe. the tbe (tris fw = 121.1, borate fw = 61.8, edta...
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