Fumarase is an enzyme in the citric acid cycle that catalyzes the conversion of fumarate to l-malate. given the fumarate (substrate) concentrations and initial velocities below, construct a lineweaver–burk plot and determine the vmax and km values for the fumarase-catalyzed reaction.
fumarate (mm) | rate (mmol 1k-1 min -1)
02.0 | 2.5
03.3 | 3.1
05.0 | 3.6
10.0 | 4.2